@brembs @albertcardona
I spy with my little eyes some nice #Platynereis larvae :)
How This Marine #Worm Can Tell Moonglow From Sunbeams
https://www.quantamagazine.org/how-this-marine-worm-can-tell-moonglow-from-sunbeams-20231219/
A marine #cryptochrome with an inverse photo-oligomerization mechanism https://www.nature.com/articles/s41467-023-42708-2
"The marine #bristleworm #Platynereis dumerilii syncs its reproductive cycle with the phases of the #moon. #Scientists recently uncovered the structure of a #protein that helps it sense #moonlight."
In the paper we managed for the first time to combine #connectomics, calcium #imaging from all neurons of a fully-mapped circuit, genetic perturbations, behaviour and computational modelling. #Platynereis for systems #neuroscience
We published a new preprint on nitric oxide (NO) signalling in #Platynereis. In the paper Kei Jokura et al. described how NO is required for UV-avoidance behaviour by mediating retrograde signalling to brain ciliary photoreceptors.
https://www.biorxiv.org/content/10.1101/2023.08.02.551600v1
#neuroscience #animals #science
Our favourite #Platynereis #opsin now in the mammalian brain as a new #optogenetic tool!
"Here we ... found that the Platynereis dumerilii ciliary opsin ... is an efficient, versatile, light-activated bistable GPCR that can suppress synaptic transmission in mammalian neurons with high temporal precision in-vivo "
Euro #EvoDevo 2024 will include the following satellite meetings:
#Amphioxus
#Platynereis
#ArthropodEvolution (that's a lot of species for a satellite meeting!)
#FishEvolution
#Crustacean
https://ecoevo.social/@EED2024/110219897240880479
Hydrostatic pressure is a dominant cue in the sea and many aquatic organisms are known to respond to changes in pressure, however the neuronal mechanisms have remained unclear.
We studied the larvae of the marine #annelid #Platynereis and found that they respond to increases in pressure by increased upward swimming.
#neuroscience
2/9
The revised version of our paper on the desmosomal #connectome of the #Platynereis larva is now out
https://elifesciences.org/articles/71231
by Sanja Jasek et al.
#volumeEM #rstat
For all electron microscopists out there:
"Crosshair, semi-automated targeting for electron microscopy with a motorised ultramicrotome"
Kimberly Meechan et al. 2022 @eLife from Yannick Schwab's lab at EMBL in collaboration with The Crick institute. https://elifesciences.org/articles/80899
Presents a new method for reliably and "selectively targeting small regions of interest in a resin block by trimming with an ultramicrotome", powered by "user-friendly software to convert X-ray images of resin-embedded samples into angles and cutting depths for the ultramicrotome."
Reviewed by three outstanding electron microscopists: Christel Genaud, Song Pang, and Michaela Wilsch-Bräuninger.
What can you do with a #CATMAID server? Say, let's look at the #Drosophila (vinegar fly, often referred to as fruit fly) larval central nervous system, generously hosted by the #VirtualFlyBrain https://l1em.catmaid.virtualflybrain.org/?pid=1&zp=108250&yp=82961.59999999999&xp=54210.799999999996&tool=tracingtool&sid0=1&s0=2.4999999999999996&help=true&layout=h(XY,%20%7B%20type:%20%22neuron-search%22,%20id:%20%22neuron-search-1%22,%20options:%20%7B%22annotation-name%22:%20%22papers%22%7D%7D,%200.6) or the #Platynereis (a marine annelid) server from the Jekely lab https://catmaid.jekelylab.ex.ac.uk/
First, directly interact by point-and-click: open widgets, find neurons by name or annotations, fire up a graph widget and rearrange neurons to make a neat synaptic connectivity diagram, or an adjacency matrix, or look at neuron anatomy in 3D. Most text–names, numbers–are clickable and filterable in some way, such as regular expressions.
Second, interact from other software. Head to r-catmaid https://natverse.org/rcatmaid/ (part of the #natverse suite by Philipp Schlegel @uni_matrix, Alex Bates and others) for an R-based solution from the Jefferis lab at the #MRCLMB. Includes tools such as #NBLAST for anatomical comparisons of neurons (see paper by Marta Costa et al. 2016 https://www.sciencedirect.com/science/article/pii/S0896627316302653 ).
If R is not your favourite, then how about #python: the #navis package, again by the prolific @uni_matrix, makes it trivial, and works also within #Blender too for fancy 3D renderings and animations. An earlier, simpler version was #catpy by @csdashm https://github.com/ceesem/catpy , who also has examples on access from #matlab.
Third, directly from a #psql prompt. As in, why not? #SQL is quite a straightforward language. Of course, you'll need privileged access to the server, so this one is only for insiders. Similarly privileged is from an #ipython prompt initialized via #django from the command line, with the entire server-side API at your disposal for queries.
Fourth, and one of my favourites: from the #javascript console in the browser itself. There are a handful of examples here https://github.com/catmaid/CATMAID/wiki/Scripting but the possibilities are huge. Key utilities are the "fetchSkeletons" macro-like javascript function https://github.com/catmaid/CATMAID/wiki/Scripting#count-the-number-of-presynaptic-sites-and-the-number-of-presynaptic-connectors-on-an-axon and the NeuronNameService.getInstance().getName(<skeleton_id>) function.
Notice every #CATMAID server has its /apis/, e.g., at https://l1em.catmaid.virtualflybrain.org/apis/ will list all GET or REST server access points. Reach to them as you please. See the documentation: https://catmaid.readthedocs.io/en/stable/api.html
In short: the data is there for you to reach out to, interactively or programmatically, and any fine mixture of the two as you see fit.